流式分析羟基磷灰石-钛生物复合材料对胎儿成骨细胞的影响

niwanmao

这是一篇关于生物材料方面的最新进展，是利用流式细胞术来分析羟基磷灰石-钛生物复合材料对人类胎儿成骨细胞的影响，主要评估了该材料对胎儿成骨细胞的凋亡、增殖、分裂指数等方面。结论是羟基磷灰石和10％wt钛复合的生物材料，对人类成骨细胞无显著影响。推荐给材料学专业的朋友。

全文下载：http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.34603/pdf
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J Biomed Mater Res A. 2013 Mar 26. doi: 10.1002/jbm.a.34603. [Epub ahead of print]

Flow cytometry analysis of human fetal osteoblast fate processes on spark plasma sintered hydroxyapatite-titanium biocomposites.
Kumar A   , Webster TJ   , Biswas K   , Basu B   .
Source
Department of Materials Science and Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, India.

Abstract
Hydroxyapatite (HA)-based biocomposites have been widely investigated for a multitude of applications and these studies have been largely driven to improve mechanical properties (toughness and strength) without compromising cytocompatibility properties. Apart from routine cell viability/proliferation analysis, limited efforts have been made to quantify the fate processes (cell proliferation, cell cycle, and cell apoptosis) of human fetal osteoblast (hFOB) cells on HA-based composites, in vitro. In this work, the osteoblast cell fate process has been studied on a model hydroxyapatite-titanium (HA-Ti) system using the flow cytometry. In order to retain both HA and Ti, the novel processing technique, that is, spark plasma sintering, was suitably adopted. The cell fate processes of hFOBs, as evaluated using a flow cytometry, revealed statistically insignificant differences among HA-10 wt % Ti and HA and control (tissue culture polystyrene surface) in terms of osteoblast apoptosis, proliferation index as well as division index. For the first time, we provide quantified flow cytometry results to demonstrate that 10 wt % Ti additions to HA do not have any significant influence on the fate processes of human osteoblast-like cells, in vitro.

PMID: 23529941