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人PBMC T细胞活化标记(续):CD71和CD95

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发表于 2013-5-21 15:09:26 | 显示全部楼层 |阅读模式

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在前一篇关于T细胞早期活化标记的帖子中(人PBMC T细胞早期活化标记及其功能介绍http://www.flowcyto.cn/bbs/thread-2322-1-1.html),我们讨论了几个早期活化标记CD69和CD40L。在T细胞受体活化后,T细胞将会表达一系列活化标记,包括细胞因子和趋化因子、粘附分子、共刺激分子、MHC Ⅱ类分子等。熟悉这些活化标记是什么,什么时候表达以及它们在正常反应和疾病状态下对于T细胞功能的起着什么作用,是研究PBMC中T细胞功能特征的重要出发点。

在本篇帖子中,我们重点讨论两个可用流式细胞术检测的中早期的T细胞活化标记:CD71和CD95

T细胞活化信号转导路径

T细胞活化信号转导路径

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综上,CD71和CD95是T细胞活化的中晚期标记,其表达失调可帮助了解不同疾病状态的T细胞功能异常。

深入阅读:
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  • Pro- and anti-apoptotic CD95 signaling in T cells. Paulsen M, Janssen O. Cell Commun Signal. 2011 Apr 8;9:7.
  • CD95 co-stimulation blocks activation of naive T cells by inhibiting T cell receptor signaling. Strauss G, Lindquist JA, Arhel N, Felder E, Karl S, Haas TL, Fulda S, Walczak H, Kirchhoff F, Debatin KM. J Exp Med 2009, 206:1379-1393.
  • Impaired interferon signaling is a common immune defect in human cancer. Critchley-Thorne RJ, Simons DL, Yan N, Miyahira AK, Dirbas FM, Johnson DL, Swetter SM, Carlson RW, Fisher GA, Koong A, Holmes S, Lee PP. Proc Natl Acad Sci U S A. 2009 Jun 2;106(22):9010-5.


发表于 2019-2-6 21:51:51 | 显示全部楼层
T细胞活化的marker:
早期  CD69
中期 CD25
晚期 HLA-DR
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