iFLEX Viper laser engine for innovativebenchtop cytometer

Super-charging nextgeneration designs

Extending the boundaries of hyperspectralflow cytometry for microbiology is their pursuit; the development of aninnovative benchtop cytometer, their stated goal: With the high-performanceiFLEX Viper lasers from Qioptic, a team of French and American researchscientists have achieved ground-breaking results.

The results confirm the capabilities of thisnew benchtop instrument for multi-parametric analysis at the single cell level.This means future improvements in instrumentation for high throughput and highresolution screening are within reach! And thanks to the success of thispioneering work, Qioptiq has been invited to demonstrate the iFLEX Viper at the5th International Conference on Analysis of Microbial Cells at the Single CellLevel, to be held in Carry-Le-Rouet, France.

Dr. Gérald Grégori of PRECYM, at the Oceanographic Centerof Marseille in France, worked with a team from the Bindley Bioscience Center,Purdue Uni­versity, USA to develop a new bench top cytometer capable ofcollecting multi­spectral data from 32 fluorescence chan­nels simultaneously.

Unlimited testing

Using the iFLEX Viper multi-laser engine Dr.Grégori was able to fire lasers with five different wavelengths(405 nm, 445 nm, 488 nm, 561 nm and 640 nm), simultaneously and sequentially,directly into the existing red laser pathway of a standard dual-lasercytometer. The output beams from all five lasers in the iFLEX Viper weredelivered collinearly by a polarization-maintaining single-mode fiber, tofacilitate the connection to the existing flow cytometer. Simultaneous emissionof all lines, power adjustment and fast switching between wave­lengths arecontrolled over a USB port, enabling optimum test procedures.

                              

The iFlex Viper enables new insights into human cell structure.

The iFlex Viper enables new insights into human cell structure.

The iFlex Viper enables new insights intohuman cell structure.

Authors:Rachel Owen and Fiona Evans, Qioptiq UK fiona.evans@qpl.qioptiq.com

Qioptiq iFLEX laser