是肺泡灌洗液吧？
听说过，但是没做过，帮你找篇参考文献，他们是做辅助性t细胞，里面有灌洗需要的液体，处理步骤，看看是否够？
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3127771/?tool=pubmed
Bronchoalveolar lavage (BAL) was performed by infusing three aliquots of 60 ml of sterile sodium chloride (NaCl), pH 7.3 at 37°C that were gently sucked back after each infusion and pooled into a container placed in iced water. The recovered fluid was immediately transported to the laboratory for analysis.
Flow cytometry analysisBAL -lymphocyte subsets were determined using flow cytometry. BAL cells were centrifuged and diluted to a final concentration of 106 cells/ml. For each test, 10 μl of antibody solution was added to 200 μl of cell suspension and allowed to bind for 30 minutes at 4°C in darkness. Red blood cells were lysed with 2 ml FACS™ Lysing solution (Becton Dickinson Immunocytometry Systems, San Jose, CA, USA) for 10 minutes at room temperature. The remaining cells were then washed by adding PBS to the tubes and centrifuged at 4°C for 10 minutes, 300 g and repeated once. Cells were thereafter fixed with 500 μl CellFIX™ (Becton Dickinson Immunocytometry Systems, San Jose, CA, USA) before analysis using a FACSCalibur™ (Becton Dickinson) flow cytometer. The lymphocyte population was gated on their physical characteristics in a region according to their characteristic forward scatter (FCS) and side scatter (SSC) profiles, as previously reported [ 6]. 3,000 total events were collected in CD3+ gate per sample
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发表于 2012-5-30 17:09:02
发表于 2012-5-30 20:49:15
