关于羟基脲同步化

山茶花村75号

流式做细胞周期有时候要同步化细胞。我现在用羟基脲同步化植物根尖的有丝分裂。羟基脲一般怎么配置啊？我要2.5 mM羟基脲（pH 6.0），直接用水配么？还是用其他溶剂？

niwanmao

供参考To bring cells to quiescence for the serum stimulation experiment, nearly confluent cells were split 1:5 and incubated overnight in DMEM containing 15% FBS. The medium was replaced with DMEM containing 0.2% FBS, and the cells were cultured for 30 h. These quiescent cells were stimulated by adding FBS at the final concentration of 15%. To bring cells to quiescence for the hydroxyurea (HU) experiment, almost-confluent cells were split 1:2 and incubated for 48 to 60 h in DMEM containing 15% FBS. Cells became quiescent due to contact inhibition during this period. These quiescent cells were released to grow by splitting 1:5 in DMEM containing 15% FBS. Three hours after splitting, HU was added to the medium at a final concentration of 0.5 mM, and cells were incubated for a further 18 h. Cells were washed twice with DMEM and refed with DMEM containing 15% FBS to release them from HU block. Cell synchrony in both experiments was assessed by flow cytometry
（http://mcb.asm.org/content/21/14/4684.full#sec-1）