用流式把血常规精细化的单管5～10色方案，一次送你5个

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临床流式实验室，外周血免疫分型是常见的需求之一。通常血常规可以进行全血细胞计数和白细胞分类，但太粗，用流式进行快速的筛查，有助于初步发现血液恶性肿瘤、感染、自身免疫疾病和/或各种免疫缺陷状态等迹象。

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结语
在血液肿瘤的筛查上，上面5个方案各有自己的特色，但肯定也都有不足之处。相信大家在自己的日常工作中，也会有其它更好的方案或补充，欢迎分享给我们。


参考文献：
van de Geijn GJ, V van Rees, N van Pul-Bom, et al. Leukoflow: multiparameter extended white blood cell differentiation for routine analysis by flow cytometry. Cytometry A; 79 (2011):694–706.
Faucher JL, C Lacronique-Gazaille, E Frebet, et al. ‘6 markers/5 colors’ extended white blood cell differential by flow cytometry. Cytometry A; 71 (2007):934–44.
Bjornsson S, S Wahlstrom, E Norstrom, et al. Total nucleated cell differential for blood and bone marrow using a single tube in a five-color flow cytometer. Cytometry B Clin Cytom; 74 (2008):91–103.
Cherian S, G Levin, WY Lo, et al. Evaluation of an 8-color flow cytometric reference method for white blood cell differential enumeration. Cytometry B Clin Cytom; 78 (2010):319–28
Melzer S, S Zachariae, J Bocsi, et al. Reference intervals for leukocyte subsets in adults: results from a population-based study using 10-color flow cytometry. Cytometry B Clin Cytom; 88 (2015):270–81