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发表于 2019-11-5 10:00:31
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在文献上看到CD69也推荐破膜后再染(CD69 is up-regulated on T cells within 6 hr of activation. Antigen-specific cytokine detection can be enhanced by demonstrating the coexpression of CD69 and the cytokine(s) of interest (Waldrop etal., 1997). The anti-CD69 MAb is typically added during the intracellular staining step, as secretion inhibitors such as BFA will prevent the surface expression of the newly produced CD69 protein.
)。另外:请教各位战友,是否遇到PMA刺激后CD3表达降低分群不明显的?如何解决,也是破膜后染吗? |
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