小鼠外周血PBMC提取

niwanmao

小憨 发表于 2015-8-2 10:20
又把老帖子翻出来问问，嘻嘻~

我也是做了好几次外周血，结果不太好，遇到点小问题，求教。

文献中用肝素抗凝：
Peripheral Blood Mononuclear Cell (PBMC) Isolation from BloodPeripheral blood from mice was collected by cardiac puncture in presence of heparin as anticoagulant.Blood (~ 1ml per mouse) was pooled according with experimental requirementsand diluted with Dulbecco's Phosphate Buffered Saline (PBS) without calcium andmagnesium (Lonza) 1x (ratio 1:1). The diluted samples were subjected to density gradientseparation on Ficoll Paque Plus (ratio 1:1) (GE Healthcare Life Sciences, Buckinghamshire,UK) and centrifuged. After centrifugation the PBMC layer was collected and washed inHank's Balanced Salt Sodium (HBSS) with phenol red without calcium and magnesium(Lonza).

链接：http://atvb.ahajournals.org/cont ... 04088-T_M_and_M.pdf

mcdfish

小憨 发表于 2015-8-2 10:20
又把老帖子翻出来问问，嘻嘻~

我也是做了好几次外周血，结果不太好，遇到点小问题，求教。

肝素钠不行吗？

mcdfish

小憨 发表于 2015-8-2 10:20
又把老帖子翻出来问问，嘻嘻~

我也是做了好几次外周血，结果不太好，遇到点小问题，求教。

肝素钠应该也行啊

niwanmao

mcdfish 发表于 2016-1-25 14:13
肝素钠应该也行啊

肝素钠应该可以的。有时候偶然的情况不一定代表不行。