细胞周期标准品对照

gcz5340

有没有朋友做细胞周期检测异倍体的，我看教材说加什么鸡红细胞做正常二倍体对照，具体是怎么操作，把鸡红细胞放在实验组的同一管里面吗？如果不是同一管里，PI的荧光强度标准不一样，没办法直接比较呀？求教求教

niwanmao

一般我平时做的时候，都会先把管子内的细胞浓度和体积调整一致，加的PI量也一致，这个荧光强度绝对不会发生大的变动。
一般是这样设置的：
1管：鸡红细胞
2管：鸡红细胞＋标本
3管：标本

鸡红细胞也可以自己配：http://www.flowcyto.cn/forum.php ... =1089&fromuid=1

设置方法：
For ploidy analysis and instrument standardization the following controls may be considered. Normal mouse thymocytes are >98% G1, containing a 2N DNA content and can be used as an external standard. Chicken red blood cells (CRBC) are 35% of normal G1 (2N) and are placed at a mean channel of 70. When used as an internal standard the 2N G1 diploid peak will present itself at channel 200. The CRBC is commercially available. The nuclei of the plant Nicotiana tabacum may be used as an external standard since it has a prominent G1 peak with a 2N DNA content.