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<b>Abstract：</b><br>
Flow cytometry has had an impact upon all areas of clinical pathology and now, in the 21st century, it is truly coming of age.
This study reviews the application of flow cytometry within clinical pathology with an emphasis upon haematology and
immunology. The basic principles of flow cytometry are discussed, including the principles and considerations of the flow-cell and hydrodynamic focusing, detector layout and function, use of fluorochromes and multicolour flow cytometry (spectral
overlap and colour compensation), alongside the strategies available for sample preparation, data acquisition and analysis,
reporting of results, internal quality control, external quality assessment and flow sorting. The practice of flow cytometry is
discussed, including the principles and pitfalls associated with leukocyte immunophenotyping for leukaemia and
lymphoma diagnosis, immune deficiency, predicting and monitoring response to monoclonal antibody therapy, rare event
detection and screening for genetic disease. Each section is illustrated with a case study. Future directions are also
discussed.
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2012 Flow cytometry in clinical pathology.pdf
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发表于 2012-3-25 19:43:59
