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发表于 2012-7-27 20:31:34
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huzengwu 发表于 2012-7-27 18:11
倪老师，这个基因是怎么用流式细胞检测的？
有现成的抗体，不是直接测基因，而是测这个基因表达的蛋白。
Technical Data Sheet
Purified Mouse Anti-Human DCC
Product Information
Material Number: 554222
Alternate Name: Deleted in Colon Cancer
Size: 0.1 mg
Concentration: 0.5 mg/ml
Clone: G92-13
Immunogen: Recombinant Human DCC (extracellular domain)
Isotype: Mouse IgG1
Reactivity: QC Testing: Human
Target MW: 175-190 kDa
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Description
One of the most common regions of allelic loss in colorectal tumors is chromosome 18, which is lost in more than 70% of carcinomas, and in almost 50% of late adenomas. This region of loss has been mapped to chromosome 18q and a gene called Deleted in Colorectal Cancer (DCC). DCC encodes an ~185 kD glycoprotein with significant homology to the neural cell adhesion molecule and other related cell surface glycoproteins. The predicted amino acid sequence of DCC cDNA consists of a 1448 amino acid (aa) long transmembrane phosphoprotein. The extracellular domain consists of 1098 amino acids and has 42% sequence homology to cell adhesion proteins of the neural cell adhesion molecule (N-CAM) family. DCC mRNA is found to be expressed in normal colonic mucosa, but its expression is reduced or absent in the majority of colorectal carcinomas. The loss of heterozygosity and subsequent alteration of DCC expression has also been observed in tumors of non-colorectal origin. G92-13 recognizes human DCC. A truncated recombinant protein containing the extracellular domain of the human DCC was used as immunogen.
不过这个抗体没有荧光，如果要做需要进行二抗标记。
http://www.bdbiosciences.com/external_files/pm/doc/tds/cell_bio/live/web_enabled/15031A_554222.pdf
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发表于 2012-7-27 09:00:34
发表于 2012-7-27 18:11:20
