|
|
亲爱的FLOWER，加入流式中文网，一起讨论，一起学习，享受更多福利吧！
您需要 登录 才可以下载或查看，没有账号？加入流式中文网
×
该方法使用的指标为Peroxidation of Leukocytes Index Ratio (PLIR)，使用的荧光染料是C11-BODIPY(581/591)（一种氧化敏感的脂质荧光探针，详见：http://www.ncbi.nlm.nih.gov/pubmed/12160930）。
PLIR指标与氧化基线水平无关，而与尿酸、FRAP负相关，能够更稳定、更方便、更真实的反映白细胞氧化还原状态。
全文免费阅读和下载地址见二楼，感谢@cccssszzz08 站友友情赞助。
＝＝＝＝原文摘要分割线＝＝＝＝
J Immunol Methods. 2013 Feb 20. pii: S0022-1759(13)00061-6. doi: 10.1016/j.jim.2013.02.005. [Epub ahead of print]
A new flow cytometry method to measure oxidative status: the Peroxidation of Leukocytes Index Ratio (PLIR).
Peluso I , Adorno G , Raguzzini A , Urban L , Ghiselli A , Serafini M .
Source
Agricultural Research Council (CRA), ex National Institute for Food and Nutrition Research (INRAN), Rome, Italy. Electronic address: i.peluso@tiscali.it.
Abstract
BACKGROUND AND AIM:
A complex relationship between immune system and metabolic pathway exists and can induce oxidative stress. The objective of this study was to design a new methodology allowing the measurement of oxidative status of leukocytes.
METHODS AND RESULTS:
We developed a flow cytometry technique, based on C11-BODIPY 581/591 staining, to evaluate peroxidation in leukocytes. We defined the Peroxidation of Leukocytes Index Ratio (PLIR) as the ratio between the damage after AAPH -induced and PMA-induced peroxidation, using Trolox as standard antioxidant. Sensitivity of the method was assessed correlating results with plasma antioxidant capacity (TRAP and FRAP), levels of endogenous antioxidants (uric acid and sulphidryls) and markers of metabolic status (cholesterol, triglycerides, glucose and insulin). PLIR measures the ratio between the resistance to exogenous and endogenous ROS injury, independently from baseline level of oxidation, which was directly correlated with plasma cholesterol on lymphocytes (0.738, p=0.029), monocytes (0.691, p=0.047) and neutrophils (0.690, p=0.047). PLIR of lymphocytes was inversely correlated with uric acid (-0.810, p=0.009) and FRAP (-0.738, p=0.029) levels. On the other hand, PLIR of monocytes was directly correlated with the total scavenger antioxidant capacity attributable to nutritional antioxidants (0.738, p=0.029), calculated as the difference between TRAP and the contribution of uric acid and sulphidryls to its value.
CONCLUSIONS:
This study reports a feasible and reproducible new flow cytometry assay for assessing the leukocytes redox status. PLIR discriminates between reducing and scavenger activities and is able to appreciate the potentially dangerous effect of uric acid on innate immune response.
PMID: 23454245
|
|
发表于 2013-3-5 21:58:13
发表于 2013-3-11 19:05:19
