TNF介导的肾小球树突状细胞聚集和活化的分析（小鼠肾脏）

niwanmao

这是一份影响因子6.61分的杂志，本文讲的是compartment-specific流式细胞术，我还想不出如何正确翻译它，从摘要中可以看得出，这个方法可以针对不同特定部位的同类指标进行分别监测，即在小鼠肾脏中，TNF可介导肾小球树突状细胞在肾小球和肾小管间质的聚集和活化，可发现MHCⅡ、CD54、CD40、CD80、CD86等指标的升高。

由于无全文，没法细读，很希望有权限的站友能够提供全文分享，一起学习研究（奖励2颗流星）。
＝＝＝＝＝原文摘要＝＝＝＝＝
Kidney Int. 2013 Mar 6. doi: 10.1038/ki.2013.46. [Epub ahead of print]
Analysis of TNF-mediated recruitment and activation of glomerular dendritic cells in mouse kidneys by compartment-specific flow cytometry.
Schwarz M   , Taubitz A   , Eltrich N   , Mulay SR   , Allam R   , Vielhauer V   .
Source
Nephrologisches Zentrum, Medizinische Klinik und Poliklinik IV, Klinikum der Universität München, Ludwig-Maximilians-University Munich, Munich, Germany.

Abstract
Renal dendritic cells (DCs) form an interstitial network contributing to inflammatory and adaptive immune responses in the kidney. The presence and functional role of DC-like glomerular CD11c+ mononuclear phagocytes is a matter of debate. Using compartment-specific flow cytometry we found that healthy mouse kidneys contained 1.3 CD11c+ cells per 100 glomeruli and these increased by 4.6-fold and 13-fold after TNF stimulation and immune complex deposition, respectively. Compartment-specific mRNA expression revealed a predominantly glomerular expression of TNF receptors, chemokines, and adhesion molecules; all upregulated after TNF exposure. Intraperitoneal TNF injection induced influx of neutrophils and mononuclear phagocytes including DC-like CD11c+ cells into both the glomerular and tubulointerstitial compartments, but reduced in TNF receptor (Tnfr) 1-deficient mice. Additionally, Tnfr2 deficiency impaired glomerular infiltration of CD11c+ cells, but not neutrophils. Interstitial CD11c+ cells infiltrated in the presence of Tnfr1 or Tnfr2. TNF exposure also induced similar maturation of glomerular and interstitial CD11c+ cells as demonstrated by increased surface expression of MHC II, CD54, and costimulatory molecules CD40, CD80, and CD86. Thus, by compartment-specific flow cytometry we could demonstrate the constitutive presence of DC-like CD11c+ mononuclear phagocytes in normal mouse glomeruli and their TNF-induced accumulation and activation.
PMID: 23466995