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在进行分选或检测时，各类蛋白质、抗体或荧光素都很容易粘附在流动室，从而导致液流不稳定和/或背景荧光增高，光散射分散，从而导致CV值增高和精确度降低。以往使用BD CLEAN和BD RINSE或含Contrad 70的氢氧化钾溶液长时间冲洗可以暂时改善CV和精确度，但很耗时。为此，2013年最新一期的cytometry A杂志上提出了一种提高FACSAria流动室清洁度的新方法。时间短，效果好。
详细步骤：
1. Execute the ‘‘Fluidic startup’’ procedure.
2. Remove the closed loop nozzle1.
3. Execute ‘‘Clean flow cell’’ procedure2 with 15% Contrad 70 solution.
4. Turn the stream on without any nozzle and activate any dummy sample from the sample list but insert in an empty tube.
5. Set the highest flow rate value. Generation of bubbles in the cuvette of the instrument should be registered within 30–60 s. The empty tube can be run as long as the bubbles are created and driven out through the cuvette, or for at least 3–5 min.
6. Shut the stream off; execute the ‘‘clean flow cell’’ procedure again with BD CLEAN solution instead of Contrad 70 solution.
7. Repeat the step 4.
8. Set the highest flow rate value. Generation of bubbles in the cuvette of the instrument should be registered within 30–60 s. Run the empty tube for at least 3–5 min.
9. Turn the stream off, insert the correct nozzle and turn the stream on again. Let the stream run for at least 3–5 min to clean the flow cell. Meanwhile execute the ‘‘Sample line backflush’’ procedure and let it run for at least 1–2min to remove the residuals of cleaning solutions from the sample line.
10. Turn the stream off and on again to remove any bubbles thatmight be left in the flow cell. ‘‘CS&T’’ procedure3 can be run now.
步骤流程图和冲洗前后的CV值对比：
步骤流程图和冲洗前后的CV值对比
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Improve_Cleaning_Aria.pdf
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发表于 2013-5-2 20:50:30
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