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[标本处理] Th1 Th2 Th17所占比例

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发表于 2013-9-29 09:50:30 | 显示全部楼层 |阅读模式
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CD4+T细胞分为TH1 TH2 TH17 TREG等细胞亚群, PMA刺激后检测各组分百分比,加起来是不是应该就与CD4的百分比差不多, 我检测后发现各组分都非常低, 是我染色没染好的缘故吗, 谢谢

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应该不是100%,主要是CD4+细胞中还可能有其它细胞存在。 看看Disturbed Th1, Th2, Th17 and Treg balance in patients with systemic lupus erythematosus文章中的图片,a是Th1、Th2、Th17占CD4+细胞的比例,可见合起来不是100%。Treg这幅图没提,但比例也很少。 Figure 1 Multiparameter flow cytometric analysis of PMA/Ca-Io induced cytokine expression in CD4+ T-cells from SLE-patients and healthy controls (HC). ...
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发表于 2013-9-29 09:50:31 | 显示全部楼层
应该不是100%,主要是CD4+细胞中还可能有其它细胞存在。
看看Disturbed Th1, Th2, Th17 and Treg balance in patients with systemic lupus erythematosus文章中的图片,a是Th1、Th2、Th17占CD4+细胞的比例,可见合起来不是100%。Treg这幅图没提,但比例也很少。

Disturbed Th1, Th2, Th17 and Treg balance in patients with systemic lupus erythematosus

Disturbed Th1, Th2, Th17 and Treg balance in patients with systemic lupus erythematosus

Figure 1 Multiparameter flow cytometric analysis of PMA/Ca-Io induced cytokine expression in CD4+ T-cells from SLE-patients and healthy controls (HC). Whole blood was stimulated with PMA/Ca-Io and analyzed for intracellular cytokine expression of IFN-γ, IL-4 and IL-17A among CD4+ T-cells. CD4+ T-cells were gated as CD3+CD8−. (a) Percentages of these T-cells are depicted. (b) The ratio of Th17/Th1 cells is shown. Horizontal lines represent the median. Red dots represent active patients. P-values were calculated using the nonparametric Mann–Whitney U-test. (c) A representative dot plot of IFN-γ, IL-4 and IL-17A expressing CD4+ T-cells upon stimulation is shown.

组织样本处理不好?流式中文网原研的魔滤®魔杵®套装,低成本解决,高质量收获
 楼主| 发表于 2013-9-29 10:22:52 | 显示全部楼层
谢谢倪老师 免疫还有这么多未知的领域等待我们探究 真是兴奋
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