原文摘录如下：Surface exposure of PS by apoptotic cells was measured by adding annexin V-FITC to the culture medium in a final concentration of 3 mg/ml. The cells were subsequently incubated for 3 min at room temperature. Detached cells were collected with the culture supernatant, pelleted by centrifugation and washed twice with culture medium in order to remove excess of annexin V-FITC. The adherent cells were rinsed twice with medium before harvesting. Cells were harvested by mechanical scraping with a rubber police man. Detached and adherent cells were finally pooled and resuspended in culture medium to a final concentration of 1×106 cells/ml. Prior to flow cytometric analysis, PI was added to a final concentration of 5 mg/ml.
发表于 2016-11-11 04:31:21
发表于 2016-11-11 07:58:55
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