找回密码
 加入流式中文网

QQ登录

只需一步,快速开始

查看: 699|回复: 1

[结构和原理] 看logicle函数原理文章的时候有段话看不懂

[复制链接]
发表于 2018-6-29 01:24:32 | 显示全部楼层 |阅读模式
悬赏1流星未解决
在看“A New ‘‘Logicle’’ Display Method Avoids Deceptive Effects of Logarithmic Scaling for Low Signals and Compensated Data”这篇文章的时候,其中有这段话是这样的,Cell populations with low mean, high variance, and approximately normally distributed fluorescence values occur commonly in various kinds of flow cytometry data. In particular, data values for cell populations that are essentially unstained or are negative for a particular dye, after fluorescence compensation, should be distributed more-or-less normally around a low value representing the autofluorescence of the cells in that data dimension. Data sets resulting from computed compensation commonly (and properly) include populations whose distributions extend below zero. (When analog compensation is used, such distributions should also appear, but the electronic implementations distort or truncate the distributions, so that negative values are suppressed.)  讲的是阴性群应该在荧光低值附近或多或少地呈正态分布,补偿正确的调节完以后,细胞群应该包含有低于0的值。
就红字这句话没看懂,能否解释一下什么意思?

文章来源

文章来源
流式中文网FlowGuard®流式专用保存液,无需冻存,稳定保护各类流式样本,从容完成实验
发表于 2018-6-29 08:12:52 | 显示全部楼层
这段话讲的是类似于BD FACS Calibur这种非数字型(模式信号)的流式细胞仪,获取的信号里面也应该会有负值,但是因为电子信号处理时,把负值丢弃了,所以在图形显示上就不出现负值了。
组织样本处理不好?流式中文网原研的魔滤®魔杵®套装,低成本解决,高质量收获
您需要登录后才可以回帖 登录 | 加入流式中文网

本版积分规则 需要先绑定手机号

手机版|流式中文网 ( 浙ICP备17054466号-2 )

浙公网安备 33038202004217号

GMT+8, 2024-12-28 18:37

Powered by Discuz! X3.5

© 2001-2024 Discuz! Team.

快速回复 返回顶部 返回列表