流式染色完不能及时上机应如何操作

爱科学

大家好，

请问如果流式染色结束后不能及时上机应该如何操作？听说可以用PFA固定，请问是一直泡在PFA里，等要上机了直接用吗？

因为在Biolegend的官网上看的protocol里这样写：
Perform fluorescence activated cell sorting (FACS), or flow cytometric analysis.
Note: If you are unable to immediately read your samples on a cytometer, keep them shielded from light and in a refrigerator set at 4-8°C. The samples should be resuspended in Cell Staining Buffer. Note that samples should not remain in a fixation buffer for extended periods of time as this can affect fluor conformation and fluorescence.  他这里的cell staining buffer就是类似PBS而已，所以我很疑惑难道不应该重悬再PFA里吗？

niwanmao

PFA 只能固定15~30分钟，必须转移到PBS里面。