要检测细胞表面蛋白的表达情况,之前用阳性率看表达情况,发现是单峰,而且药物处理后,峰有位移,所以改用MFI。看到一篇文献中给出了这样的公式:The increase in mean fluorescence intensity (ΔMFI) was calculated as follows: (MFI with specific mAb – MFI with isotype control) / MFI with isotype control. The relative MFI (rMFI) value was calculated to compare the difference between the ΔMFI of a specific treatment and control as follows: ΔMFI of specific treatment / ΔMFI of control treatment × 100%. 我也想按这种方法进行一下分析。
请问,mean值是看P1门的,还是应该看P2门的呢?因为没有明显的阴阳两个峰,是不是应该看P1门的mean值呢?谢谢大家!
还有请问,图2的单参数直方图的拖尾可能是什么原因造成的呢?谢谢!
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