关于小鼠皮肤组织流式细胞术制备问题

luzhouyu

想问问大家对小鼠皮肤组织流式细胞术有没有了解呀？之前没有做过
有没有制备单细胞悬液的经验，组织怎么消化比较好

niwanmao

Mouse	Mouse	Epidermal	Neutral Protease: 0.5%Collagenase Type 4: 0.1%	DMEM
Mouse	Mouse	Epithelial Stem	Trypsin: 0.25%Collagenase Type 1: 0.4%	HBSS
Mouse	Mouse	Dermal fibroblasts	Collagenase: 400 u/ml	AminoMAX II
Mouse	Mouse	Epidermal and dermal	Collagenase Type 4: 0.18%Collagenase/Dispase: 0.18%	PBS
Mouse	Mouse, 2-3 day	Dermal fibroblasts	Collagenase Type 2: 0.04%Trypsin: 0.025%	DME
Mouse	Mouse	Dermal	Collagenase Type 3: 0.3%Deoxyribonuclease I: 0.0005%	HBSS
Mouse	Mouse, neonatal	Dermal	Collagenase: 0.35%Deoxyribonuclease I: see reference	PBS
Mouse	mouse	Dermal fibroblasts	Trypsin: 0.25%Collagenase Type 1: 0.25%	DMEM
Mouse	Mouse, neonatal	Microvascular endothelial	Neutral Protease: 0.005%Collagenase Type 1: 4%	DMEM
Mouse	Mouse, male 6-24 week	Skin side population	Collagenase Type 4: 0.2%Neutral Protease: 1.2 u/ml	PBS
Mouse	Mouse	Ear epidermal	Trypsin: 0.1%Collagenase: 0.2%	PBS
Mouse	Mouse, 3-7 month	Fibroblasts, mesangial, smooth muscle	Trypsin: 0.25%Collagenase: see referenceSoybean Trypsin Inhibitor: .05%	DMEM

参见：最完整的实体组织酶消化指导手册
https://www.flowcyto.cn/bbs/thread-5834-1-1.html
(出处: 流式中文网)

luzhouyu

niwanmao 发表于 2023-3-28 13:06
参见：最完整的实体组织酶消化指导手册
https://www.flowcyto.cn/bbs/thread-5834-1-1.html
(出处: 流 ...

谢谢！！！

luzhouyu

niwanmao 发表于 2023-3-28 13:06
参见：最完整的实体组织酶消化指导手册
https://www.flowcyto.cn/bbs/thread-5834-1-1.html
(出处: 流 ...

老师 或者可以问问有没有皮肤组织免疫细胞的制备呀

niwanmao

luzhouyu 发表于 2023-4-9 21:34
老师 或者可以问问有没有皮肤组织免疫细胞的制备呀

那个帖子里面，各物种、各类组织，均有。