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发表于 2023-4-14 10:01:17
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未用过，但我相信标记技术是很成熟的。
问了下Bing AI，回答如下，不知道是否准确，仅供参考用。
There are many different antibody labeling kits available for flow cytometry, and the best one may depend on your specific application and preferences. However, based on the search results, some of the most popular and widely used kits are:
- Alexa Fluor™ Antibody Labeling Kits¹³: These kits provide a convenient means to label antibodies or proteins (>40 kDa) with as many as nine different colored dyes. Conjugated antibodies produce lower background fluorescence with less nonspecific binding and are ideal for multiplexing in a variety of applications. Each kit contains sufficient dye to conjugate 100 µg per reaction and enough reagent for five reactions.
- SiteClick™ Antibody Labeling Kits²: These kits achieve highly efficient, site-specific, and reproducible labeling by targeting the carbohydrate domains present on essentially all IgG antibodies, regardless of isotype and host species. Depending upon the label, the resulting SiteClick R-PE, Qdot, or Biotin conjugated antibody can be used in flow cytometry, fluorescence imaging, or Western blot detection. Each kit contains everything needed to label 100 µg of IgG antibody with an easy-to-follow, step-by-step protocol.
I hope this helps you find the best antibody labeling kit for your needs.😊
Source: Conversation with Bing, 2023/4/14
(1) . https://bing.com/search?q=flow+cytometry+antibody+labeling+kit.
(2) Alexa Fluor™ Antibody Labeling Kits - Thermo Fisher Scientific. https://www.thermofisher.com/order/catalog/product/A20181.
(3) SiteClick™ Antibody Labeling Kits - Thermo Fisher Scientific. https://www.thermofisher.com/order/catalog/product/S10467.
(4) Antibody Labeling Kits | Thermo Fisher Scientific - US. https://www.thermofisher.com/us/en/home/life-science/antibodies/antibody-labeling.html.
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发表于 2023-4-13 14:28:00
