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发表于 2012-9-1 15:44:47
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清言 发表于 2012-9-1 11:42
站长,您好,您这张图是做的正常人对照来检测B27,请问是否每次做B27都需要找个正常人做对照呢(在没有标准 ...
哦,这张图是我从实用流式细胞术图谱一书摘录的是,并非是我工作中做出来的。而我们这边只开展白血病免疫分型、白血病残留病灶检测、PNH、干祖细胞分析等项目,所以只能从一些guideline上对你的问题进行回答,希望有相关项目开展的站友可以出来回答一下。 @huzengwu @stef1981 @haven_t
澳大利亚一个协会出的guideline上有这么一段关于质控的话:
6. QUALITY ASSURANCE
6.1. Each laboratory should determine mean channel of fluorescence cut-offs at which HLA-B27 is excluded and demonstrated. The nature of the assay is such that in some samples the mean channel of fluorescence falls within these cut-offs (‘grey area’), these samples would need for further testing. To avoid false positive assignment due to cross-reactivity of monoclonal antibodies, HLA-B27 screening should require the use of two different mAb clones (14-15).
每个实验室都需要确定区分HLA-B27阳性、阴性的平均荧光强度截断值。如果某些标本的平均荧光强度刚好落在该截断值范围内,这些标本需要进一步重新测试。为了防止单克隆抗体交叉反应导致的假阳性,建议使用至少两种不同克隆的单克隆抗体进行HLA-B27筛查。
6.2. The laboratory should belong to and participate in a recognised external quality assurance program as provided by RCPA Quality Assurance (program HLA B27).
实验室需要参加RCPA质控组织的外部质控程序(流式中文网注:这个在我们中国目前似乎还没有,淋巴细胞亚群的质控似乎CDC已经在搞了,不过似乎实施有点难度。)
6.3. The laboratory should determine the level of test variability by preparing and analysing a positive sample at least six times over a period of 48 hrs after venipuncture.
6.4. If possible set up an HLA-B27 positive sample once a week or after post servicing of instrument, especially when setting up this assay. Controls cells frozen from typed donors, stabilised whole blood controls or cell line available from commercial manufacturers may be used. Samples demonstrating HLA-B27 status from the previous day’s run may also be used as control material.
如果可能,至少一周做一次HLA-B27的阳性标本。健康供者的冻存细胞、商业化的外周血质控品或细胞株都可以。前一天做的已经确诊的HLA-B27样本也可以做为质控对照。(流式中文网注:这句话应该是你想要的答案 @清言)
PDF附件见:
HLAB27_reviewed_2009.pdf
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使用流式中文网资料阅读器在线阅读,免流星,内容完全一样(使用说明)
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