DCC基因在不同肿瘤组织中的表达

niwanmao

DCC基因（概念转自百度百科 http://baike.baidu.com/view/598799.htm ）
　　DCC (Deleted in colon cancer)
　　DCC基因最初从直肠癌中得到鉴定,位于18q21.1，含有1.4Mbp和29个外显子。其表达产物为190kD的跨膜磷蛋白，膜外有1100个氨基酸，膜内有324个氨基酸。DCC的氨基酸序列与NCAM及其它相关的细胞表面糖蛋白具有同源性，这提示DCC功能的丢失可能导致细胞间接触、粘附能力下降，从而提高癌细胞的转移能力。
　　癌变时DCC基因存在多种改变，如等位基因丢失、5’端同源性丧失、内含子及外显子点突变、杂合缺失 (loss of heterozygosity，LOH)和DCC低表达等。在食管癌的原发灶和转移淋巴结中分别检测DCC基因的B、C、D、E 4个外显子，结果只在转移淋巴结发现有保守密码子的错义点突变。另DCC的等位缺失的频率则直接与转移程度有关，即转移得越远，等位缺失的频率越高。有人报道DCC的等位缺失与直肠癌和肺癌远器官转移有关。Hiroshi等研究表明DCC等位缺失还存在于低分化和中等分化的鳞形细胞癌中，却不存在于高分化的鳞癌中，这说明DCC的等位缺失还与细胞分化有关，提示DCC功能缺失可导致癌细胞转移及去分化。此外，在鳞癌的转移复发灶而未在原发灶中发现18qLOH的结果提示，位于18q的抑癌基因在肿瘤转移过程中起重要作用。

DCC基因在不同肿瘤组织中的表达

huzengwu

倪老师，这个基因是怎么用流式细胞检测的？

niwanmao

huzengwu 发表于 2012-7-27 18:11

                               
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倪老师，这个基因是怎么用流式细胞检测的？

有现成的抗体，不是直接测基因，而是测这个基因表达的蛋白。

Technical Data Sheet
Purified Mouse Anti-Human DCC
Product Information
Material Number: 554222
Alternate Name:  Deleted in Colon Cancer
Size:  0.1 mg
Concentration: 0.5 mg/ml
Clone:  G92-13
Immunogen: Recombinant Human DCC (extracellular domain)
Isotype: Mouse IgG1
Reactivity: QC Testing: Human
Target MW: 175-190 kDa
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Description
One of the most common regions of allelic loss in colorectal tumors is chromosome 18, which is lost in more than 70% of carcinomas, and in almost 50% of late adenomas.  This region of loss has been mapped to chromosome 18q and a gene called Deleted in Colorectal Cancer (DCC). DCC encodes an ~185 kD glycoprotein with significant homology to the neural cell adhesion molecule and other related cell surface glycoproteins. The predicted amino acid sequence of DCC cDNA consists of a 1448 amino acid (aa) long transmembrane phosphoprotein. The extracellular domain consists of 1098 amino acids and has 42% sequence homology to cell adhesion proteins of the neural cell adhesion molecule (N-CAM) family. DCC mRNA is found to be expressed in normal colonic mucosa, but its expression is reduced or absent in the majority of colorectal carcinomas.  The loss of heterozygosity and subsequent alteration of DCC expression has also been observed in tumors of non-colorectal origin.  G92-13 recognizes human DCC. A truncated recombinant protein containing the extracellular domain of the human DCC was used as immunogen.


不过这个抗体没有荧光，如果要做需要进行二抗标记。
http://www.bdbiosciences.com/external_files/pm/doc/tds/cell_bio/live/web_enabled/15031A_554222.pdf