磷酸盐缓冲液配制

niwanmao

标题：磷酸盐缓冲液配制

PBS (phosphate-buffered saline)
0.23 g NaH2PO4 (anhydrous; 1.9 mM)
1.15 g Na2HPO4 (anhydrous; 8.1 mM)
9.00 g NaCl (154 mM)
Add H2O to 900 ml
If needed, adjust to desired pH (usually 7.2 to 7.4) with 1 M NaOH or 1 M HCl
Add H2O to 1 liter
Filter sterilize
Store indefinitely at 4°C
Without pH adjustment, pH will generally be ~7.3.
Some laboratories find it convenient to prepare PBS at 10× concentration and dilute this
to make the working solution. PBS can also be purchased commercially in powder form.

回答者：抗体

现在确实方便了，有粉剂买，一小包可以配10升，才几块钱！！

2009年8月12日 0:54