巨噬细胞磁珠分选

samly0

请问各位朋友有没有谁用磁珠分选过大鼠的肺组织巨噬细胞，选用什么标志？或者有没有什么现成的磁珠分选试剂盒？

gcz5340

小鼠有F4/80，不知道大鼠有没有。

niwanmao

不知道这篇文章里面的这个表格对你是否有帮助？

文献：http://respiratory-research.com/content/7/1/4

表

Antibody	Antigen/Cell population	Expression	Differences
1A29	ICAM-1 (CD54)	medium	medium
1C7	mononuclear phagocytes (CD68 ?)	medium	medium
3.2.3.	NKR-P1 (CD161)	weak	no
3A12	PECAM-1 (CD31)	weak	no
5F10	VCAM-1	no
ART18	IL-2 receptor	no
ART65	IL-2 receptor	no
ED2	macrophage subset (no monocytes)	no
ED3	macrophage subset (no monocytes)	no
ED4	macrophages	medium	no
ED7	macrophage subset (CD11/CD18; CR3)	medium	strong
ED8	macrophage subset (CD11/CD18; CR3)	medium	small
ED9	macrophage subset (SIRPα, CD172a)	strong	medium
KIM2R	mature tissue macrophages	no
MAR3	macrophage subset	no
Ox2	CD200	no
Ox26	transferrin receptor (CD71)	no
Ox3	MHC-II (I-A like)	weak	small
Ox4	MHC-II (I-A like)	weak	small
Ox41	macrophages, DCs, PMNs (SIRP)	no
Ox50	hyaluronic acid receptor (CD44)	medium	small
Ox52	activated monocytes
Ox6	MHC-II (RT1.B; I-A)	weak	medium
Ox62	DC subpopulation	no
Ox8	CD8α	no
Ox85	L-selectin (CD62L)	no
RM-1	monocytes/macrophages/DCs/PMNs	strong	small
RM-4	all macrophages (no monocytes)	medium	strong
RMA	macrophage subset (120 kDa antigen)	medium	medium
RP-1	neutrophiles (intracellular)	no
RP-3	neutrophiles (intracellular)	no
W3/13	leukosialin (CD43)	no
WT/1	LFA-1 (CD11a)	weak	no

samly0

收到了，谢谢哈，很有帮助

liuxin

gcz5340 发表于 2012-10-11 13:32

                               
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小鼠有F4/80，不知道大鼠有没有。

您做过小鼠肝脏中F4/80的分选或者流式检测吗？

gcz5340

liuxin 发表于 2012-10-17 09:48

                               
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您做过小鼠肝脏中F4/80的分选或者流式检测吗？

没有，只做过培养的小鼠巨噬细胞的鉴定。