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用流式细胞术检测浓缩血小板中的细菌污染

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发表于 2012-11-7 13:24:30 | 显示全部楼层 |阅读模式

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这篇文章是一本书中的一个章节,比较新,是今年最近刚出的,这里面讲到了分别用流式细胞术和实时PCR方法检测浓缩血小板的细菌污染,这为拓宽流式细胞术的应用范围又做出了贡献。不过可惜的是,似乎里面用的都是德国一家公司(AES Chemunex GmbH, Bruchsal, Germany)的流式细胞仪和配套试剂,流式细胞仪的参数没问题,其它机器也有,就不知道这几个试剂是否也适用,期待该公司来给大家发试用装:)。

Detection of Bacterial Contamination in Platelet Concentrates Using Flow Cytometry and Real-Time PCR Methods
Tanja Vollmer , Knut Kleesiek , and Jens Dreier
Abstract
Despite considerable advances in the safety of blood components based on the application of highly sensitive and speci fi c screening methods to minimize the viral infection risk, the prevention of transfusion-associated bacterial infection remains a major challenge in transfusion medicine. In particular, platelet concentrates represent the greatest infectious risk of transfusion-transmitted bacterial sepsis. The detection of bacterial contamination in platelet concentrates has been implemented in several blood services as a routine quality control testing. Although culture is likely to remain the gold standard method of detecting bacterial contamination,
the use of rapid methods is likely to increase and play an important role in transfusion medicine in the future. In particular, fl ow cytometric methods and nucleic acid ampli fi cation techniques are powerful tools in bacterial screening assays. Compared to culture-based methods, the combination of high sensitivity and speci fi city, low contamination risk, ease of performance, and speed has made those technologies appealing alternatives to conventional culture-based testing methods.

流式细胞术检测细菌

流式细胞术检测细菌


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