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尽管有不少基因标记用来分离乳腺肿瘤干细胞，但是无法与其肿瘤性和转移性相关，为此，本文作者选择了10 种干细胞标记，检测了1种正常乳腺细胞株（MCF10-A）和4种人乳腺癌细胞株（MCF-7、MDA-MB-231、MDA-MB-435、Hs578-T），最后发现CD90和CD14可作为鉴定恶性乳腺癌细胞株肿瘤干细胞的标记。<br><br>有全文下载权限的站友可跟帖附上全文，奖励5颗流星。<br><br><h2 style="font-size: 16px; color: rgb(34, 34, 34); font-family: arial, sans-serif;">Differential expression of CD90 and CD14 stem cell markers in malignant breast cancer cell lines.</h2><div class="meta" style="font-size: 12px; max-width: 60em; line-height: 1.5714; color: rgb(34, 34, 34); font-family: arial, sans-serif;"><h3 class="os" style="font-size: 12px; float: left; text-indent: -9999em; height: 0px;">Authors</h3><div id="slav"><div class="auth">Lobba AR, Forni MF, Carreira AC, Sogayar MC.</div></div><h3 class="os" style="font-size: 12px; float: left; text-indent: -9999em; height: 0px;">Journal</h3><p class="j" style="margin-top: 0.7em; margin-bottom: 0.7em;">Cytometry A. 2012 Dec;81(12):1084-91. doi: 10.1002/cyto.a.22220. Epub 2012 Oct 22.</p><h3 class="os" style="font-size: 12px; float: left; text-indent: -9999em; height: 0px;">Affiliation</h3><p class="aff" style="margin-top: 0.7em; margin-bottom: 0.7em;">Biochemistry Department, Chemistry Institute, Cell and Molecular Therapy Center (NUCEL), University of São Paulo, 05508-000 São Paulo, Brazil.</p></div><div class="ab" style="margin-top: 1.5em; margin-bottom: 1.5em; max-width: 60em; line-height: 1.5714; color: rgb(34, 34, 34); font-family: arial, sans-serif;"><h3 class="no_b_m" style="font-size: 12px;">Abstract</h3><p class="no_t_m" style="margin-bottom: 1.4em;">The recently emerged concept of cancer stem cell (CSC) has led to a new hypothesis on the basis for tumor progression. Basically, the CSC theory hypothesizes the presence of a hierarchically organized and relatively rare cell population, which is responsible for tumor initiation, self-renewal, and maintenance, in addition to accumulation of mutation and resistance to chemotherapy. CSCs have recently been described in breast cancer. Different genetic markers have been used to isolate breast CSCs, none of which have been correlated with the tumorigenicity or metastatic potential of the cells, limiting their precise characterization and clinical application in the development of therapeutic protocols. Here, we sought for subpopulations of CSCs by analyzing 10 judiciously chosen stem cell markers in a normal breast cell line (MCF10-A) and in four human breast cancer cell lines (MCF-7, MDA-MB-231, MDA-MB-435, and Hs578-T) displaying different degrees of metastatic and invasiveness potential. We were able to identify two markers, which are differentially expressed in nontumorigenic versus tumor cells. The CD90 marker was highly expressed in the malignant cell lines. Interestingly, the CD14 molecule displayed higher expression levels in the nontumorigenic cell line. Therefore, we demonstrated that these two markers, which are more commonly used to isolate and characterize stem cells, are differentially expressed in breast tumor cells, when compared with nontumorigenic breast cells. © 2012 International Society for Advancement of Cytometry.</p>Copyright © 2012 International Society for Advancement of Cytometry.</div><div class="meta" style="font-size: 12px; max-width: 60em; line-height: 1.5714; color: rgb(34, 34, 34); font-family: arial, sans-serif;"><div class="ids" style="margin-top: 1em; margin-bottom: 1em;"><h3 style="font-size: 12px; font-weight: normal; display: inline; margin-right: 0.5em;">PMID</h3> <span style="margin-right: 0.5em;">23090904 [PubMed - in process]</span></div><div class="ids" style="margin-top: 1em; margin-bottom: 1em;">Full text: <a href="http://dx.doi.org/10.1002/cyto.a.22220" style="color: rgb(68, 17, 153);">John Wiley & Sons, Inc.</a></div></div><p></p> |
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发表于 2012-12-6 21:02:07
发表于 2020-9-2 00:36:52
