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Biomark Med.2月份的文章。该杂志当前最新影响因子2.63分。
目的：流式细胞术通过检测锚蛋白缺陷来鉴定阵发性睡眠性溶血性贫血（PNH）已成为诊断PNH的标准方法。然而，选择合适的标记非常重要。
材料与方法：CD59、GPHA（CD235a）、CD33、CD15、FLAER、CD16、CD24、CD14
结果：该方案可有效鉴定和定量骨髓和外周血中的红细胞、粒细胞、单核细胞群PNH克隆，在外周血标本的红细胞和粒细胞群中，最低可检测出0.01％的PNH克隆。对比实验提示，FLAER更适合于骨髓标本的PNH克隆检出。
（流式中文网翻译，如需转载，请保留出处和链接。）
全文参见本帖6楼，感谢@tanweifeng 站友提供
＝＝＝＝原文摘要分割线＝＝＝＝＝
Biomark Med. 2013 Feb;7(1):99-111. doi: 10.2217/bmm.12.80.
Diagnosis of paroxysmal nocturnal hemoglobinuria in peripheral blood and bone marrow with six-color flow cytometry.
Yang HS , Yang M , Li X , Tugulea S , Dong H .
Source
Esoterix Genetic Laboratories LLC, 521 W 57th Street, New York, NY 10019, USA.
Abstract
Aim: Identification of paroxysmal nocturnal hemoglobinuria (PNH) by detecting a glycophosphatidylinositol-anchored defect by flow cytometry is presently the standard method of choice for diagnosing PNH. However, the selection of suitable markers will be critical and significantly affect the determination and quantification of PNH clones in various cell lineages.
Materials & methods: In this study, we investigated the performance of various immunophenotypic markers including CD59, GPHA (a clustered antigen, CD235a), CD33, CD15 and fluorescent aerolysin (FLAER) combined with CD16, CD24 and CD14 in a PNH panel using six-color flow cytometry.
Results: The results strongly indicate that these markers can collectively and effectively identify and quantify PNH clones in erythrocyte, granulocyte and monocyte populations derived from peripheral blood and bone marrow (BM). A sensitivity threshold as low as 0.01% in identifying PNH clones in erythrocyte and granulocyte populations from peripheral blood is achieved by this panel in a series dilution assay. In addition, a direct side-by-side comparison between BM and peripheral blood from the same patients suggests that the FLAER PNH test is capable of identifying to PNH clones in BM specimens.
Conclusion: The data support the premise that a six-color flow cytometry PNH panel using the combination of CD59, CD235a, CD33, CD15, FLAER, CD16, CD24 and CD14 can enhance and improve the current methods used in diagnosis and management of PNH by specifically identifying PNH clones in the erythrocyte, granulocyte and monocyte population.
PMID: 23387491
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发表于 2013-2-26 11:24:18
发表于 2013-2-28 23:18:12
