流式细胞术检测大肠杆菌吸附T2噬菌体

niwanmao

这是一篇2013年3月份J Virol Methods. （影响因子2.01）的文章，讲的是流式细胞术检测大肠杆菌吸附T2噬菌体个例研究。对此领域我不是很熟悉，但是对流式的应用这么广感觉非常好奇，推荐给各位研究病毒的朋友。
全文链接：http://www.sciencedirect.com/sci ... i/S0166093413000566
＝＝＝＝＝原文摘要＝＝＝＝＝
J Virol Methods. 2013 Mar 11. pii: S0166-0934(13)00056-6. doi: 10.1016/j.jviromet.2013.02.007. [Epub ahead of print]
Phage adsorption to bacteria in the light of the electrostatics: A case study using E. coli, T2 and flow cytometry.
Zemb O   , Manefield M   , Thomas F   , Jacquet S   .
Source
INRA, UMR1289 Tissus Animaux Nutrition Digestion Ecosystème et Métabolisme, F-31326 Castanet-Tolosan, France. Electronic
Abstract
The addition of sodium chloride to freshwater or diluted minimal salt medium increases the adsorption of T2 phages on Escherichia coli. For the first time the adsorption in diluted minimal salt medium was measured by counting unadsorbed phages (i.e. free particles) using flow cytometry, allowing a gentle separation between adsorbed and unadsorbed phages. Flow cytometry was able to detect weakly adsorbed phage that remained undetected using classical centrifugation-based methods and this allowed us to show that increasing ionic strength enhances the phage adsorption to its bacterial host with an extremely low detection limit. A key result was that the adsorption in high ionic strength (i.e. 100mM) reached 4.5±0.1 10-5mL/min which is 1,400 fold higher than previously reported values.<ABS-P>In order to understand the mechanism underpinning such a weak phage adsorption, the zeta potentials and the diffusion coefficient of the particles were measured by dynamic light scattering. The bacterial cells and the phages had zeta potentials between -60mV and -10mV and -30mV and -10mV respectively. The diffusion coefficient of the phage was 2.8±0.4 10-12 m2/s corresponding to a hydrodynamic radius of 104±15nm. However significant adsorption occurs in conditions where the DLVO theory predicts that minimal encounter, suggesting that forces other that electrostatic repulsion and Van der Waals interaction (e.g. potential impurities, particle shape and other biological characteristics) are likely to interplay.
Crown Copyright © 2013. Published by Elsevier B.V. All rights reserved.
PMID: 23499611

boiledtea

谢谢推荐，立刻下来看看。。