应该不是100%,主要是CD4+细胞中还可能有其它细胞存在。
看看Disturbed Th1, Th2, Th17 and Treg balance in patients with systemic lupus erythematosus文章中的图片,a是Th1、Th2、Th17占CD4+细胞的比例,可见合起来不是100%。Treg这幅图没提,但比例也很少。
Disturbed Th1, Th2, Th17 and Treg balance in patients with systemic lupus erythematosus
Figure 1 Multiparameter flow cytometric analysis of PMA/Ca-Io induced cytokine expression in CD4+ T-cells from SLE-patients and healthy controls (HC). Whole blood was stimulated with PMA/Ca-Io and analyzed for intracellular cytokine expression of IFN-γ, IL-4 and IL-17A among CD4+ T-cells. CD4+ T-cells were gated as CD3+CD8−. (a) Percentages of these T-cells are depicted. (b) The ratio of Th17/Th1 cells is shown. Horizontal lines represent the median. Red dots represent active patients. P-values were calculated using the nonparametric Mann–Whitney U-test. (c) A representative dot plot of IFN-γ, IL-4 and IL-17A expressing CD4+ T-cells upon stimulation is shown.
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