在流式细胞术中排除背景荧光的影响（综述）

niwanmao

标题：在流式细胞术中排除背景荧光的影响（综述）
这是一篇综述，详细描述了自发荧光、荧光重叠、非特异性荧光等背景荧光的产生原因和解决对策，无论对于临床流式还是科研流式都非常有帮助。

链接：http://onlinelibrary.wiley.com/doi/10.1002/cyto.b.20485/full

Considerations for the control of background fluorescence in clinical flow cytometry


Abstract
Accurate measurement of antigen-positive cells by flow cytometry can be hampered by background fluorescence of antigen-negative cells and other particles (e.g., debris). This article focuses on three major causes of background (autofluorescence, spectral overlap, and undesirable antibody binding) by reviewing individual aspects of flow cytometric measurements that contribute to these causes. The appropriate use of controls facilitates a thorough understanding of these contributing factors as well as the development of robust cell labeling protocols intended for routine flow cytometric analysis. We present a set of recommendations that enables the user to develop an optimized cell labeling protocol that minimizes background and maximizes the ability to reliably distinguish between a positive and a negative population of cells. These recommendations are also intended to augment existing guidelines designed to aid in the formulation of a consensus regarding the utility of flow cytometry for the analysis of clinical samples. © 2009 Clinical Cytometry Society