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楼主 |
发表于 2014-12-18 21:20:25
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那倪老师我根据您的意见再修改了一下您看这样的图片还有问题么？
Method: Percentage of Immature platelet fraction is the proportion of reticulated platelets within the total number of platelets. We conducted the analysis based on previous studies. In detail, EDTA anti-coagulated blood were centrifuged at 120g for 4 minutes. Then the supernatant, which is platelet rich plasma, was harvested. 10 ul of supernatant and 20ul CD41-PE antibody (BD, Biosciences) with 1ml thiazole orange (TO, Sigma-Aldrich) were mixed in the dark for 15 minutes. Analysis of the samples was performed with a flow cytometer (Beckman-Coulter, Miami, FL, USA). The platelet population in forward (versus side) scattering was confirmed as the population of CD41-PE positive events in the cytogram and was gated for this platelet population area. Based on RNA staining by TO, TO positivity was made by measuring both forward light scatter and green (540 nm) fluorescence using logarithmic amplification among CD41 positive events.
Figure legend: Gating strategies for IPF% analysis. Selected patients from non-survivor group (figure A and B) and survivor group (figure C and D) were demonstrated. Figure A and Figure C showed the FSC/CD41-PE plot for platelets. Figure B and Figure D showed the gating of CD41+ cells. The CD41+ population was backgated into the TO/SSC plot.CD41+TO+ platelets with appropriated size were viewed as IPF
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发表于 2014-12-17 19:03:19
发表于 2014-12-17 21:33:42