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发表于 2015-4-15 10:27:10
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BD官方有一份关于细胞因子检测的FAQ，里面第3页清楚地写了两者的区别：
The major difference between these two inhibitors is where they work within a cell. BD GolgiStop (monensin) works by accumulation of protein at the endoplasmic reticulum (ER) stage, while BD GolgiPlug (brefeldin) works with accumulation of protein at the golgi complex. The use of these inhibitors leads to an enhanced ability to detect cytokine-producing cells by intracellular flow cytometry.
简译之，就是说两者的作用部位不同，GolgiStop作用于内质网，将蛋白阻滞在内质网，而GolgiPlug则作用于高尔基体，使蛋白积累在高尔基体内。两者同用是没有问题的。
免费在线阅读FAQ：
https://flowcyto.cn/bbs/forum.php?mod=attachment&aid=Nzg5NXw4NWM2ZjNkNXwxNzMyMjY5OTEwfDB8
下载FAQ PDF文件（1颗流星）：
cytokineFAQ.pdf
(253.69 KB, 下载次数: 13, 售价: 1 流星)
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发表于 2015-4-14 20:45:05
