各位老师，你好，有个科研相关的问题想请教大家：
A single cell suspension of PB-MNCs was stained for
lineage markers (CD45R/B220, TER-119, TCR β, TCR
γδ, Gr-1 and CD11b) with antibodies conjugated to
fluorescein isothiocyanate (FITC), for CD45 with APCconjugated
antibody, and for Sca-1 with PE-conjugated
antibody (all from BD Biosciences Pharmingen, San
Diego, USA). Next, cells were incubated for 30 min
on ice, washed and fixed in 1% formaldehyde. After
a final washing step, the cells were analyzed by
fluorescence-activated cell sorting (FACS Aria II, BD
Biosciences, USA). At least 105 events were acquired and
analyzed using Cell Quest software (BD Biosciences).
The cell populations of Sca-1+CD45−Lin− cells and
Sca-1+CD45+Lin− (enriched in HSCs) were analyzed as
shown in Figure 2 (panel A and B), and expressed as
percentages of total events.
在文献看到这样的操作，我想做相同的实验。现在已按上面买了对应的抗体，现在问题是我的panel设计是lineage markers 的六种抗体先混合好再染色，还是分别染六管不同的lineage markers？
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发表于 2016-4-3 14:45:53
发表于 2016-4-3 23:54:47