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标题：关于树突状细胞表面标志物 今天帮人做实验，用CD80、CD83、CD86看树突状细胞激活情况，这倒好分析，但如何圈出树突状细胞就存疑问了，她说她的细胞是经梯度密度离心找出PBMC的，又帖壁吸附了，只剩树突状细胞了，但我看前向侧向角好像还有不少的淋巴及中性粒，。印象中CD14+算单核的标志物，查了文献有说那树突的标志是CD11c+CD1a+CD14-，y有点晕，到底哪些标志物是特异的还经刺激培养后又不会有太大的变化，期待中。。。。。。 回答者：niwanmao 给你转一段文字，不翻译了，直接看英文更好理解。对你了解树突状细胞非常有帮助：
Dendritic cells (DCs) are immune cells and form part of the mammalian immune system. Their main function is to process antigen material and present it on their surface to other cells of the immune system. Dendritic cells are present in small quantities in tissues that are in contact with the external environment, mainly the skin (where they are often called Langerhans cells) and the inner lining of the nose, lung, stomach and intestines. They can also be found at an immature state in the blood. Once activated, they migrate to the lymphoid tissues where they interact with T cells and B cells to initiate and shape the immune response. At certain development stages they grow branched projections, the dendrites, that give the cell its name. However, these do not have any special relation with neurons, which also possess similar appendages. Immature dendritic cells are also called veiled cells, in which case they possess large cytoplasmic \'veils\' rather than dendrites.
下面是一些树突状细胞的相关标记及其表达意义：
ADAM19 (MADDAM)
BDCA-2
CD1a
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a marker of immature DCs, plays a role in tumorigenesis. PMID: 16159438
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a human epidermal dendritic cell marker related to class I MHC molecules. PMID: 2784820
CD11c
CD21
CD83
CD86
CD208
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CD208 DC lysosomal-associated protein is a marker of activated human dendritic cells.PMID: 16899762
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Dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208, a member of the lysosomal associated membrane protein (LAMP) family, is specifically expressed by human DCs on activation. PMID: 14982840
CLIP-170/restin
Clusterin
DC-LAMP (CD208)
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a member of the lysosomal-associated membrane protein (LAMP) family, is specifically expressed by human dendritic cells (DC) upon activation and therefore serves as marker of human DC maturation. PMID: 12938238, PMID: 14982840
DEC-205
Estrogen Receptor-alpha
Fascin
HLA-DR
MADDAM (metalloprotease and disintegrin dendritic antigen marker)
NLDC-145
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a dendritic cell specific marker NLDC. PMID: 3265929
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recognizes DEC-205, a 205-kDa integral membrane glycoprotein. PMID: 7758125, PMID: 7553896
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NLDC-145 detects an antigen (DEC-205) expressed at high levels by mouse dendritic cells (DCs) and thymic epithelial cells. PMID: 7553896
S-100
2009年8月12日 0:54 | 
发表于 2010-8-1 21:04:33
发表于 2012-8-22 21:43:21
