树突状细胞在FSC-SSC点图的位置？

niwanmao

标题：树突状细胞在FSC-SSC点图的位置？
树突状细胞做流式，横坐标是FCS，纵坐标是SSC，读取1万个细胞，但是不知道应该选取哪个范围的作为树突状细胞？望大虾解答

回答者：niwanmao
首先说一句，提问请注意标题尽量简洁，然后在问题描述中讲的详细点。
然后来解答你的问题：
一般的FSC是比较大的，SSC中等。
例如下图（引用自：http://www.rbej.com/content/6/1/46/figure/F1）：

子宫颈粘膜树突状细胞各亚群

Quantification of dendritic cell subsets in cervical mucosa by flow cytometric analysis. (A) Introduction of an acquisition gate (R1) on the forward-scatter (FSC) versus side-scatter (SSC) profile to select mononuclear cell population. (B) cervical DCs were identified within gate R2 as lineage fluorescein isothiocyanate (FITC) cocktail (contains anti-CD3, CD14, CD16, CD19, CD20, and CD56) negative and HLA-DR positive. (C-D) Detection of HLA-DR+ CD11c+ myeloid DCs and HLA-DR+CD123+ plasmacytoid DCs respectively. FSC, forward scatter; SSC, side scatter; PerCP, peridin chlorophyll protein; PE, phycoerythrin; APC, allophycocyanin
2009年8月12日 0:54