4色法检测CLL中的ZAP70表达

niwanmao

A Single Tube, Four-Color Flow Cytometry Assay for Evaluation of ZAP-70 and CD38 Expression in Chronic Lymphocytic Leukemia
Nagwa M. Hassanein, MD1,*, Kathryn R. Perkinson, HTL(ASCP)2,Felisa Alcancia, MT(ASCP)2, Barbara K. Goodman, PhD1,J. Brice Weinberg, MD3 and Anand S. Lagoo, MD, PhD1

1From the Department of Pathology and
2Clinical Flow Cytometry Laboratory, Duke University Medical Center and
3Department of Medicine (Division of Hematology), Duke University Medical Center and Veterans Affairs Medical Center, Durham, NC.
Address reprint requests to Dr Lagoo: Dept of Pathology, Box 3712, Duke University Medical Center, Durham, NC 27710.

Abstract

We describe a simple and robust flow cytometry assay for ZAP-70 and CD38 expression. The steps required to validate this assay in a clinical flow cytometry laboratory are described. Two criteria were used to characterize ZAP-70 expression into positive, negative, and indeterminate categories and applied to 111 cases of chronic lymphocytic leukemia (CLL) resulting in 29.7% positive, 56.8% negative, and 13.5% indeterminate cases. A sensitivity-specificity crossover plot between ZAP-70 and CD38 suggested a cutoff of 12.5% for defining CD38 positivity. ZAP-70+ cases were significantly more likely to be at a higher clinical stage and, together with CD38+ cases, were more likely to have unmutated IgVH. However, for individual patients, the concordance between these markers was not perfect. It may be necessary to evaluate several prognostic markers simultaneously in CLL, and availability of convenient assays for ZAP-70 and CD38 is desirable for optimal clinical decision making.

全文链接：http://ajcp.ascpjournals.org/content/133/5/708.full

niwanmao

总结一下这篇文章的ZAP70表达水平判断方法。
标准1：CLL细胞和正常B淋巴细胞的ZAP70 MFC比例≥2
标准2：CLL细胞的ZAP70表达率≥40%

如果上述两个标准均符合，则判断为阳性。
如果只符合其中一个标准，则判断为可疑（Indeterminate）
如果两个标准均不符合，则判断为阴性。


CD38的阳性标准为≥30%。

aninawang

nilaoshi，可以提供全文吗？

niwanmao

nilaoshi，可以提供全文吗？
aninawang 发表于 2010-9-7 00:47

                               
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