你是对的，虽然这个分子是胞膜的，但还是需要破膜染，因为我在文献《The macrophage scavenger receptor CD163.》里面找到了原因：
CD163 is like most members of the SRCR family, expressed as a membrane bound protein, but until now is the only scavenger receptor that has been reported to be actively shed from the cell surface.
你是对的，虽然这个分子是胞膜的，但还是需要破膜染，因为我在文献《The macrophage scavenger receptor CD163.》里面找到了原因：
CD163 is like most members of the SRCR family, expressed as a membrane bound protein, but until now is the only scavenger receptor that has been reported to be actively shed from the cell surface.
他们的结果比较好，里面的方法学可参考一下：
To generate human macrophages, CD14+monocytes were cultured in complete HEPES-buffered RPMI 1640 supplemented with 10% FBS and either 10 ng/ml GM-CSF or 50 ng/ml M-CSF for 7 days, as differentiation with these stimuli results in M1 or M2 macrophage polarization, respectively. Macrophage polarization was verified using flow cytometry to measure expression of cell surface markers CD206 (clone: 15–2), CD163 (clone: GHI/61), and HLA-DR (clone: L243) and by ELISA analysis of secreted cytokines post-LPS stimulation.
发表于 2019-8-19 16:44:49
发表于 2019-8-19 16:44:50
