|
发表于 2020-8-26 20:34:48
|
显示全部楼层
活细胞用CD45(白细胞共同抗原)和CD68(泛巨噬细胞标志物)双标分出总巨噬细胞,然后用CD86(M1标志物)和CD163( M2标记)区分出M1和M2。
https://pubmed.ncbi.nlm.nih.gov/27805599/
(Rubio-Navarro A, Guerrero-Hue M, Martín-Fernandez B, et al. Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry. J Vis Exp. 2016;(116):54599. Published 2016 Oct 18. doi:10.3791/54599) |
|