常有FLOWER在公众号后台问M1和M2的表型特点,如何检测,或者在论坛内提问。
在此,将小鼠和人单核、巨噬细胞M1、M2、Kupffer细胞、DC表型汇总表分享如下,大家可根据需要,选择鉴别能力强的指标
表格来源:Cossarizza A, Chang HD, Radbruch A, et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies. Eur J Immunol. 2017 Oct;47(10):1584-1797.
人巨噬细胞M1和M2的区分,大多用CD11c和CD206;小鼠的,可参考下面这篇文献里面的指标,可以用CD206,但在分群不佳的时候,也可结合结合CD206和一些M2的功能指标:
图片来源:Haloul, M., Oliveira, E.R.A., Kader, M. et al. mTORC1-mediated polarization of M1 macrophages and their accumulation in the liver correlate with immunopathology in fatal ehrlichiosis. Sci Rep 9, 14050 (2019). https://doi.org/10.1038/s41598-019-50320-y