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发表于 2021-3-26 10:47:54
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在Much More than M1 and M2 Macrophages, There are also CD169+ and TCR+ Macrophages里面有这么一段话对TCR+巨噬的描述：
Regarding macrophage cellular subpopulations, several publications have reported the presence of both humans and murine TCR+ macrophages. The TCR-αβ was described to be expressed by peripheral blood monocytes and in vitro by activated monocyte-derived macrophages. Using an in vitro model, Beham et al. showed that TCRβ locus rearrangement and expression of Vβ repertoires in the myeloid lineage occurred during the early phase of macrophage differentiation. TCR+ macrophages have the ability to release CCL2 and possess high-phagocytosis capacity. These cells express molecules such as ZAP70, LAT, Fyn, and Lck, necessary for TCR signaling on lymphocytes; however, their concentration was different when macrophages received IL-4 or IFN-γ stimuli that determine different macrophages’ polarization. Interestingly, during M. tuberculosis infection, which derived pathology involves granuloma formation, macrophages at the inner epithelioid cell zone of caseous granulomas are TCR+ macrophages. Neutralization of TNF (cytokine necessary for host resistance to M. tuberculosis) in patients with lung tuberculosis, suppressed the expression of the CD3 ζ-subunit on TCR+ macrophages, becoming unstable to form the complex TCR-CD3, and the number of TCR+ macrophages decreased. Anti-TNF treatment is also associated with granuloma disorganization and reduced CCL2 expression, thus suggesting that TNF is a regulator of TCRαβ expression on macrophages (165).
可见，主要是用巨噬细胞标记（如CD45、CD14、CD11b、HLA-DR等）再加上TCRβ抗体。至于实际应用方案，你在该文里面的参考文献中找找看。 |
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发表于 2021-3-26 10:47:53
