Brefeldin和Monensin有什么区别？两者中有一种就可以了吗？

xyhongjin

Brefeldin和Monensin都是高尔基阻断剂，有何区别？在以PMA及Ionomycin刺激T细胞产生细胞因子时，可以阻断细胞因子外排至细胞外，这时二者有一种就够了吗？

niwanmao

相对来说，Brefeldin A更好。参见参考文献：http://www.ncbi.nlm.nih.gov/pubmed/11449408

Cytometry. 2001 Jun 15;46(3):172-6.
Evaluation of monensin and brefeldin A for flow cytometric determination of interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha in monocytes.Schuerwegh AJ, Stevens WJ, Bridts CH, De Clerck LS.
SourceDepartment of Immunology, Allergology and Rheumatology, University of Antwerp, Antwerp, Belgium.

AbstractFlow cytometry has become a powerful technique to measure intracellular cytokine production in lymphocytes and monocytes. Appropriate inhibition of the secretion of the produced cytokines is required for studying intracellular cytokine expression. The aim of this study was to compare the capacity of cytokine secretion inhibitors, monensin and brefeldin A, in order to trap cytokine production (interleukin-1 beta [IL-1beta], IL-6, tumor necrosis factor-alpha [TNF-alpha]) within peripheral blood monocytes. A two-color flow cytometric technique was used to measure intracellular spontaneous and lipopolysaccharide (LPS)-stimulated IL-1beta, IL-6, and TNF-alpha production in monocytes (CD14+) of whole blood cultures. The viability of monensin-treated monocytes was slightly lower than that of brefeldin A-inhibited monocytes, as measured with propidium iodide (PI). The percentage of IL-6 and TNF-alpha-producing monocytes after 8 h of culture without stimulation revealed significant lower values for monensin-treated than for brefeldin A-treated monocytes. The percentages for stimulated cells did not differ. The spontaneous intracellular production in molecules of equivalent soluble fluorochrome units (MESF) of IL-1beta, IL-6, and TNF-alpha after 8 h of culture was higher in brefeldin A than in monensin-inhibited monocytes. The LPS-stimulated intracellular production of IL-1beta, IL-6, and TNF-alpha was increased in brefeldin A-inhibited monocytes. In conclusion, for flow cytometric determination of intracellular monocytic cytokines (IL-1beta, IL-6, and TNF-alpha), brefeldin A is a more potent, effective, and less toxic inhibitor of cytokine secretion than monensin.

PMID: 11449408  [PubMed - indexed for MEDLINE]

xyhongjin

niwanmao 发表于 2011-11-2 22:07

                               
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相对来说，Brefeldin A更好。参见参考文献：http://www.ncbi.nlm.nih.gov/pubmed/11449408

Cytometry. 200 ...

谢谢倪老师！

chujindong

xyhongjin 发表于 2011-11-2 22:21

                               
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谢谢倪老师！

Brefeldin A更好，这个结论应该是公认的吧。monensin也是不错的，看了很多相关文献，用monensin的似乎更多，因为monensin便宜，而Brefeldin A要贵得多。两个有一个就行了。