间期细胞染色体FLOW-FISH

niwanmao

2012年8月29日的BLOOD发表了一篇文章，讲的是利用间期细胞来做7号染色体单体的FLOW-FISH，改变了常规染色体只能做分裂相的缺点，有点意思，推荐给大家。不过由于vysis的探针还是很贵，个人觉得临床应用可行性不大，但该技术应该还是有一定的前景的。

Interphase Chromosome Flow-FISH
Keyvan Keyvanfar, Jason Weed, Prashanth Swamy, Sachiko Kajigaya, Rodrigo T. Calado and Neal S. Young

Abstract
A 2-day method using flow cytometry and fluorescence in situ hybridization (FISH) for interphase cells was developed to detect monosomy 7 cells in myelodysplastic syndrome patients. The method, Interphase Chromosome Flow-FISH (IC Flow-FISH), involves fixation of leukocytes from blood, membrane permeabilization, hybridization of cellular DNA with peptide nucleic acid probes with cells intact, and analysis by flow cytometry. Hundreds to thousands of monosomy 7 cells were consistently detected from 10-20 mL of blood in patients with monosomy 7. Proportions of monosomy 7 cells detected in IC Flow-FISH were compared with results from conventional cytogenetics; identification of monosomy 7 populations was verified with fluorescence-activated cell sorting; and patient and donor cells were mixed to test for sensitivity. IC Flow-FISH allows for detecting monosomy 7 without requiring bone marrow procurement or the necessity of metaphase spreads, and wider applications to other chromosomal abnormalities are in development.

正常标本和7号单体标本

＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝＝
PDF附件见： blood-2012_interphase_chromosome_Flow_FISH.pdf (740.28 KB, 下载次数: 7, 售价: 2 流星)

2012-9-24 09:18 上传

点击文件名下载附件

，下载需要2颗流星（为什么？）

congou

我想做Y染色体的flow-fish，能否同时做细胞表面抗原染色？谢谢！

niwanmao

congou 发表于 2014-4-9 10:15
我想做Y染色体的flow-fish，能否同时做细胞表面抗原染色？谢谢！

做是能做，可在固定之前做，但由于本方法中的甲醇等固定剂对细胞形态破坏很大，故也会影响表面抗原最后的表达。一般不建议做。