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循环肿瘤细胞（CTC）的计数对于临床上实体肿瘤的预后判断和预测非常重要。CellSearch是一种免疫磁珠CTC分选和计数系统，已应用于临床，ImageStream结合了流式和荧光显微镜技术，在CTC计数中也有着很大的潜力。本文影响因子1.99分，比较了这两项技术对于CTC计数的能力，使用的是PANC-1肿瘤细胞，混入7.5ml正常人外周血。在ImageStream分析前，另外使用了抗EpPCAM做免疫磁珠分选。CD45和CK抗体用于鉴别肿瘤细胞和白细胞。结果发现两种技术检测的结果相似，但均与参考值存在显著差异，ImageStream在CTC数量低时，精确度较低。这提示ImageStream平台对于初发时CTC计数有潜在意义，但对于后续的监测，则需要进一步优化。
（流式中文网原创翻译总结）
全文参见二楼，感谢@dragonhzqsmmu 站友提供全文。
＝＝＝＝＝＝原文摘要＝＝＝＝＝＝＝
APMIS. 2013 Mar 20. doi: 10.1111/apm.12061. [Epub ahead of print]
Imaging cytometry for counting circulating tumor cells: comparative analysis of the CellSearch vs ImageStream systems.
López-Riquelme N , Minguela A , Villar-Permuy F , Ciprian D , Castillejo A , Alvarez-López MR , Soto JL .
Source
Molecular Genetics Laboratory, Elche University Hospital, Elche, El Palmar, Spain.
Abstract
Circulating tumor cell (CTC) enumeration is important clinically for identifying prognostic and predictive factors in patients with solid cancers. The CellSearch device (Veridex) is an immunomagnetic CTC selection and enumeration system used in clinical practice. The ImageStream (Amnis) combines the strengths of flow cytometry and fluorescent microscopy in a single platform and has potential application for CTC counting. The performance in CTC enumeration was compared between the ImageStream and CellSearch systems. Various numbers of PANC-1 tumor cells were spiked into 7.5 mL of peripheral blood from a healthy donor. Before cell analysis by the ImageStream, tumor cell enrichment was performed by immunomagnetic selection with anti-EpPCAM. Anti-CD45 and anti-CK markers were used to discriminate between tumor cells and leukocytes. The ratios of tumor cells recovered from each dilution were calculated for both methods. The Wilcoxon rank test was applied to compare the results of the two methods and the reference value. The results of the two tested methods differed significantly from the reference value, but did not differ between them. Nevertheless, lower level of trueness and precision was observed in ImageStream when fewer numbers of CTCs were analyzed. Our results suggest that ImageStream platform for CTC enumeration has a potential value for the early diagnosis of disseminated disease, but needs an improvement of precision for the enumeration of low number of CTC.
PMID: 23510386
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发表于 2013-3-31 21:14:17
发表于 2013-6-5 12:45:51
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