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[结构和原理] 有没有对激光器比较了解的?

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发表于 2013-6-4 21:39:32 | 显示全部楼层 |阅读模式
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现在的流式机器都越来越小,像BD的C6、Verse,密理博的guava、美天旎的MACSQuant的激光器都很小,相对于Calibur以及Canto II、Aria的大块头laser简直就是袖珍型的,但是看激光功率以及荧光的激发效率一点也不逊色。有没有高手对激光器很了解的,难道激光发展到现在已经不需要那种大的激光器?

发表于 2013-6-4 22:15:16 | 显示全部楼层
好像看到过类似资料,但是当时一眼扫过,没仔细读-_-!  明天找找电脑上的文档看。
做到这么小是一点没有问题的。
这些小的应该都是固体激光器吧   @haven_t  是不是比较懂?
 楼主| 发表于 2013-6-4 22:28:43 | 显示全部楼层
是啊,难道那些氦氖激光机都该淘汰了。笔记本越小越贵,小流式却便宜。
发表于 2013-6-4 22:52:51 | 显示全部楼层

固体激光器全名叫dpss,即diode pump solid state laser,这种玩意儿是应该比氦氖这些稀有气体便宜。笔记本就不同了,那是真正的微型化。
发表于 2013-6-5 08:21:35 | 显示全部楼层
niwanmao 发表于 2013-6-4 22:15
好像看到过类似资料,但是当时一眼扫过,没仔细读-_-!  明天找找电脑上的文档看。
做到这么小是一点没有问 ...

我也不太懂激光器,相对于我以前使用Vantage的激光器有1米多长,而且要带个水冷设备,Canto的已经算很小了。

现在淘宝上有些激光笔能点燃火柴,标称的功率不比流式的激光器小哦。
发表于 2013-6-5 14:11:46 | 显示全部楼层
gcz5340 发表于 2013-6-4 22:28
是啊,难道那些氦氖激光机都该淘汰了。笔记本越小越贵,小流式却便宜。

郭版,给你一篇cytometry A上的技术文章参考,讲固态激光的:
Diode and DPSS lasers emitting a variety of wavelengths are now commonly incorporated into flow cytometers, greatly increasing our capacity to excite a wide variety of fluorochromes. Until recently, however, virtually no practical technology existed for generating yellow or orange laser light for flow cytometry that was compatible with smaller instrumentation. In this study, we evaluate several new solid state laser systems that emit from the 570 to 600 nm as excitation sources for flow cytometry. DPSS 580, 589, and 592 nm sources were integrated into a cuvette-based flow cytometer (BD LSR II) and a stream-in-air cell sorter (FACSVantage DiVa), and used to excite a variety of yellow, orange, and red excited fluorochromes, including Texas Red, APC, and its tandem conjugates, and the genetically encoded red fluorescent protein HcRed and the more recently developed Katushka. All laser sources were successfully incorporated into the indicated flow cytometry platforms. The yellow and orange sources (particularly 592 nm) were ideal for exciting Texas Red, and provided excitation of APC and its tandems that was comparable to a traditional red laser source, albeit at higher power levels than red sources. Yellow and orange laser light was optimal for exciting HcRed and Katushka. Practical yellow and orange laser sources are now available for flow cytometry. This technology fills an important gap in the laser wavelengths available for flow, now almost any fluorochrome requiring visible light excitation can be accommodated.

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发表于 2013-6-11 07:10:41 | 显示全部楼层
也许很多技术已经不是什么问题了,就是有没有人去做而已,只不过我们隔行如隔山不了解罢了,希望将来的流式越来越小,功能越来越强大,价格越来越便宜。

据说,国内正在研发流式
发表于 2013-6-11 07:14:53 | 显示全部楼层
mybiosciences 发表于 2013-6-11 07:10
也许很多技术已经不是什么问题了,就是有没有人去做而已,只不过我们隔行如隔山不了解罢了,希望将来的流式 ...

国内我知道的就有三家,有点战国时代的味道
发表于 2013-6-11 08:51:41 | 显示全部楼层
那太好了,什么东西只有中国开始制造了,价格才能拉下来
发表于 2013-6-11 09:06:02 | 显示全部楼层
mybiosciences 发表于 2013-6-11 08:51
那太好了,什么东西只有中国开始制造了,价格才能拉下来

其它两家价格不清楚,至少其中一家真的如你所想,打价格战
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