抗体染色体积的问题。

Kimbryant

1.  Aliquot 100 µL of whole blood to each tube.
2.  [Optional] Pre-incubate the cells with 20 μL of Human Fc Receptor Binding Inhibitor
Purified per 100 µL of blood for 10-20 minutes at 2-8oC or room temperature prior to
staining.
3.  Combine the recommended quantity of each primary antibody in an appropriate volume
of Flow Cytometry Staining Buffer so that the final volume of antibody mix is 50 µL. Add
to cells and pulse vortex gently to mix.  Incubate for 20-30 minutes at room temperature.
Protect from light.
这个是指全血的反应体系是150微升吗？100+50，抗体是不是也要加量，说明书上是100微升5微升，那这个150微升是不是就要7.5微升？这个事eBioscience全血表面抗体标记的说明书

niwanmao

就100ul全血，其它抗体的体积不用计较。