有没有用CFSE染色做混合淋巴细胞反应的protocol？

niwanmao

标题：倪老师，请问有没有用CFSE染色做混合淋巴细胞反应的protocol？如题？以及如何用modfit LT 软件分析？
回答者：niwanmao

Modfit LT分析淋巴细胞增殖的步骤（官方转载）：

=> Launch ModFit LT and pass through the opening dialogs.

=> Using the Open Data command, navigate to the Samples\Proliferation folder in the application folder.

=> Select “CFSE sample.fcs” and click Open.

                               
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=> Choose “P3: FL1-H-CFSE” from the parameter list, and click OK.

                               
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=> Leave the Gate checkboxes unchecked and click OK.

=> From the Analysis menu, choose Proliferation Wizard, and then Create or edit model.

=> In the Proliferation Wizard dialog, click the Generations tab.

=> Choose the “Floating” model option from the drop-down list.

                               
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=> In the Proliferation Wizard dialog, click the Other tab.

=> Enable the Export options checkboxes: “Export partition table” and “Prompt for file name”.

These options will cause the program to create the export file after analysis and allow you to enter a name for the export file.

=> Finally, click the Analyze button to perform the analysis.

When the analysis is complete, the Save dialog will be displayed. You can save the file to any location.

=> For this tutorial, save the export file in the same folder as the data file.

When you choose the Export option for the Proliferation Wizard, the program determines the boundaries between adjacent generations and writes that information to the export file. The boundaries are the points of intersection between the Gaussian components in the model. An example is shown below.

                               
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2009年8月12日 0:54