全血也要100 微升吗?这是eBioscience的表面抗体标记流程,是150微升的体系吗
1. Aliquot 100 µL of whole blood to each tube.
2. [Optional] Pre-incubate the cells with 20 μL of Human Fc Receptor Binding Inhibitor
Purified per 100 µL of blood for 10-20 minutes at 2-8
o
C or room temperature prior to
staining.
3. Combine the recommended quantity of each primary antibody in an appropriate volume
of Flow Cytometry Staining Buffer so that the final volume of antibody mix is 50 µL. Add
to cells and pulse vortex gently to mix. Incubate for 20-30 minutes at room temperature.
Protect from light.