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发表于 2013-7-23 09:22:24
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samly0 发表于 2013-7-23 09:00
没做个CFSE和PI双染，楼主要是做了，能不能上几张图？想看看图是怎样的 ...
找张典型的给你看看，不过这张图是7-AAD：
As all target cells were initially stained green, the entire population can be counted in R3=R2+R1. After the effector cells and red live/dead stain were added, all the dead target cells were stained red, shifting them up the Y-axis (R2), clearly distinguishing them from the live target cells (R1). (Live effector cells are at lower left, dead effector cells are in upper left). Using the Basic Cytotoxicity Test, the analysis would be complete (and R2 would be the maroon line above). However, ICT's Total Cytotoxicity Kit includes an additional caspase reagent to concurrently assess apoptosis (which is the blue line above). To complete the analysis using the Total Cytotoxicity Test, further analyze R1 and R2 to isolate apoptotic cells.
来源：http://www.immunochemistry.com/products/total-cytotoxicity-test-assay-kit.html
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楼主: 风行
发表于 2019-2-25 22:08:28
