流式细胞术目前已知的应用范围，欢迎补充和讨论

niwanmao

这里列出了我能搜集到的流式细胞术目前已知的应用范围（38类），欢迎补充和讨论。

• Immunophenotyping / Intracellular antigens measurement
• DNA/RNA: cell cycle, aneuploidy, endoreduplication, kinetics
• DNA base ratios
• Chromatin structure
• Apoptosis (DNA degradation, mitochondrial membrane potential, permeability changes,  caspase activity)
• Membrane potential
• Membrane fluidity
• Membrane fusion/runover
• Intracellular calcium (ions) flux
• Intracellular pH
• Sulfhydryl groups/glutathione
• Cell viability
• Cell tracking and proliferation
• Intracellular reactive oxygen species (Oxidative burst)
• Cell proliferation
• Cell enumeration
• Cell volume and morphological complexity
• Cell pigments (f.ex. chlorophyll or phycoerythrin)
• Drug delivery
• Multidrug resistance (MDR)
• Phagocytosis
• Pathogen-host cell adherence
• Differentiation
• Identification of “stem cells”
• Reticulocyte, platelet etc analysis
• Microparticles analysis
• Assessing infection/transfection levels
• Monitoring of the electropermeabilization of cells
• Cytotoxicity assay
• Enzymatic activity
• Cell activation
• Protein-protein interactions (FRET, split-GFP)
• Protein modifications, phospho-proteins
• Activation of signalling pathways
• Cytokine Secretion
• Sorting (f.ex sperm sorting for sex preselection)
• Karyotyping
• Telomere length
  

virginforest

倪老师，这些实验要是能把每种的原理和方法整理出来岂不是功德无量{:soso_e113:}

niwanmao

virginforest 发表于 2014-10-20 14:33
倪老师，这些实验要是能把每种的原理和方法整理出来岂不是功德无量 ...

大家一起动手，每人整理一样呗：）